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Elisa has been used as a diagnostic tool in medicine, plant pathology, and biotechnology, as well as a quality control check in various industries It is a method of quantifying an antigen immobilized on a solid surface. In the most simple form of an elisa, antigens from the sample to be tested are attached to a surface.
Elisa is a laboratory technique that detects certain antibodies, antigens and other substances in your blood, pee or other bodily fluid This guide explains what an elisa (short for enzyme linked immunosorbent assay) is, the procedures involved, types of elisa, detection options and results Laboratory scientists use this technique for several medical tests — from diagnosing infections to confirming pregnancy.
Other names, such as enzyme immunoassay (eia), are also used to describe the same technology.
This guide covers standard curve fitting, od interpretation, and troubleshooting tips to help you improve elisa assay performance and data reliability. Elisas are a type of immunoassay that are commonly used to quantify levels of a specific target within a sample Samples routinely used in elisas include serum, plasma, cell culture supernates, cell lysates, saliva, tissue lysates, and urine. The elisa procedure is a cornerstone technique in immunology, infectious disease, biomarker discovery, and qc labs
When optimized, an elisa assay delivers sensitive, quantitative data on proteins, antibodies, and other analytes with impressive reliability Carefully following each of the elisa procedure steps helps maintain a strong signal, controlled background, and low variability.
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