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Direct microscopic visualization of the malarial parasite on the thick and/or thin blood smears has been the gold standard for malaria diagnosis. Blication data microscopy for the detection, identification and quantification of malaria parasites on stained thick and thin blood films in research settings (version 1.0) If possible, the whole thick film should be scanned.
Malaria parasites concentrate below the granulocyte layer in the tube A thick film is prepared by placing a small drop of blood on a slide then spreading it in a circular motion The qbc method is reported to have a good sensitivity for detection of malaria parasites, and has also been applied (albeit to a lesser extent) to other parasites such as trypanosomes, microfilaria and babesia spp.
Thin and thick blood films serve different purposes in malaria diagnosis
Thin films allow visualisation of a monolayer of cells (rbc, wbc, and platelets), along with any intracellular or extracellular parasites. There is no single correct choice of staining, essentially the thin film has many advantages in terms of ease of preparation, staining and identification In contrast the thick malarial film offers a single but significant advantage That of superior sensitivity for parasite detection.
Once the diagnosis is established—usually by detecting parasites in the thick smear—the microscopist can examine the thin smear to determine the malaria species and the parasitemia, or the percentage of the patient's red blood cells that are infected with malaria parasites. Performing a parasite count on a thick fi lm and calculating parasite density Place the glass slide on the microscope, with the label to the left However, these methods lack sensitivity to detect low parasitaemia.
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