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Roles of dna polymerases and other replication enzymes If two dna molecules have matching ends, they can be joined by the enzyme dna ligase. Leading and lagging strands and okazaki fragments.
Dna polymerases are the enzymes that build dna in cells It works alongside dna helicase and other enzymes to ensure accurate replication During dna replication (copying), most dna polymerases can “check their work” with each base that they add
This process is called proofreading
If the polymerase detects that a wrong (incorrectly paired) nucleotide has been added, it will remove and replace the nucleotide right away, before continuing with dna synthesis 1. Dna polymerase is the enzyme responsible for synthesizing new dna To do this, dna polymerase moves along a template strand and adds complementary nucleotides one at a time to a growing dna molecule. Polymerase chain reaction, or pcr, is a technique to make many copies of a specific dna region in vitro (in a test tube rather than an organism)
Pcr relies on a thermostable dna polymerase, taq polymerase, and requires dna primers designed specifically for the dna region of interest. Telomeres act as caps that protect the internal regions of the chromosomes, and they're worn down a small amount in each round of dna replication In this article, we'll take a closer look at why telomeres are needed, why they shorten during dna replication, and how the enzyme telomerase can be used to extend them. Transcription is an essential step in using the information from genes in our dna to make proteins.
Specifically, rna polymerase builds an rna strand in the 5' to 3' direction, adding each new nucleotide to the 3' end of the strand.
Dna replication is a precise process where dna unwinds and splits into two strands Each strand then serves as a template for a new dna molecule The leading strand is built continuously, while the lagging strand is built in fragments, called okazaki fragments. Dna polymerase iii plays a crucial role in dna replication, proofreading and correcting errors
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